WebJun 1, 2007 · Staining was then developed by FITC-Tyramide according to the manufacturer's instructions. After MWT, an anti-GFAP antibody (1:500; Dako, Carpinteria, CA) that was also raised in rabbit was applied for 1 hr at room temperature. Secondary detection was the same as for the TH immunostaining, except that AlexaFluor 594 … WebThis particular kit features AlexaFluor 488 tyramide (496/524 ex/em), detected using a standard Green/FITC/GFP filter cube. This kit also features poly-HRP-conjugated goat anti-mouse IgG secondary antibody. ...
Fitc Labeled Tyramide PerkinElmer Bioz
WebTyramide signal amplification (TSA), sometimes called Catalyzed Reporter Deposition (CARD), is a highly sensitive method enabling the detection of low-abundance targets in fluorescent immunocytochemistry … WebJun 26, 2016 · The tyramide conjugates were detected as follows: TNP-tyr was detected with rabbit anti-DNP (RADNP 1:100; DAKO) and FITC-labeled swine anti-rabbit IgG (SWAR-FITC 1:80; DAKO); DIG-tyr with FITC-labeled rabbit anti-DIG (RADIG-FITC 1:100; Boehringer); BIO-tyr with FITC-labeled avidin (Av-FITC 1:500; Vector, Brunschwig … inconsistency\u0027s ug
Formaldehyde‐based whole‐mount in situ hybridization method …
WebTyramide signal amplification (TSA) has proven to be a particularly versatile and powerful enzyme amplification technique with improved assay sensitivity. TSA is ... readily detected with the standard FITC filter set. AT A GLANCE Protocol summary 1. Fix/permeabilize/block cells or tissue 2. Add primary antibody in blocking buffer 3. Add HRP ... WebTyramide signal amplification (TSA) has proven to be a particularly versatile and powerful enzyme amplification technique with improved assay sensitivity. TSA is based on the ability of HRP, in the presence of low concentrations of hydrogen peroxide, to convert labeled tyramine-containing substrate into an oxidized, highly reactive free radical ... Web(B) Western blot (WB) after TSA labeling with either tyramide-biotin or tyramide-FITC. Cell lysate without TSA reaction and a TSA reaction without primary antibody were included … inconsistency\u0027s u7