WebWash the column with 5 to 10 column volumes of binding buffer or until no material appears in the flowthrough. Save the flowthrough for SDS-PAGE analysis to check for any loss … WebThe Human CHST4/GST-3 ELISA Kit is a solid-phase sandwich Enzyme-Linked Immunosorbent Assay (ELISA) designed to detect and quantify the level of human CHST4 in cell culture supernatants, plasma, and serum. ... Allow Wash Buffer Concentrate (20X) to reach room temperature and mix to redissolve any precipitated salts. ...
Pull-down assays - Sigma-Aldrich
WebBugBuster ® GST•Bind™ Purification Kit 70794 Millipore BugBuster ® GST•Bind™ Purification Kit Download Zoom Affinity purification of GST fusion proteins MSDS … WebThe yield of GST-tagged proteins is highly variable, ranging from 1 mg/l to 10 mg/l. The yield depends on various parameters, such as nature of the tagged protein, the host cell, and … contact pawn stars
Glutathione Resin User Manual - Takara Bio
WebGlutathione-based affinity purification of GST-tagged fusion proteins is easily done at either small, medium or large scales to produce microgram, milligram or gram quantities. At 26 kDa, GST is considerably larger than … WebPreparing the buffers Use high-purity water and chemicals, and pass all buffers through a 0.45 µM filter before use. Alternative binding buffers: 5 to 40 mM imidazole can be included in the binding buffer to reduce nonspecific binding of … contact patty murray office