Webanti-HEL antibody HyHEL-10 Fv fragment. HyHEL-10 has six Tyr residues in its antigen-binding site, which were systemati-cally mutated to Phe and Ala using site-directed …

Molecular recognition of lysozyme by monoclonal antibodies.

WebAlanine scanning mutagenesis of the HyHEL-10 paratope of the HyHEL-10/HEWL complex demonstrates that the energetically important side chains (hot spots) of both partners are … Web24 sep. 1999 · Crystal structure of anti-Hen egg white lysozyme antibody (HyHEL-10) Fv-antigen complex. Local structural changes in the protein antigen and water-mediated … prathi roju pandage songs download

Three antibodies, HyHEL-8 (HH8), HyHEL-10 (HH10), and HyHEL …

Web1 mei 1993 · The D101GHEL mutant is bound to HyHEL-10 as tightly as wild-type enzyme, but the delta delta Gdissoc is increased by about 2.2 kcal (9.2 kJ)/mol for the larger residues in this position. HEL variants with lysine or histidine replacements for arginine at position 21 are bound 1.4-2.7 times more tightly than those with neutral or negatively charged amino … Web13 dec. 2002 · To study the role of hydrogen bonding via interfacial water molecules in protein-protein interactions, we examined the interaction between hen egg white … Web1 mrt. 2013 · HyHEL-10 CDRL3 peptide (HyHEL-10-L3) was cyclized as well for negative control. Although technical issues prevented the evaluation of HEL binding by GST-fused peptides using SPR, the synthetic cyclized peptides are not GST fused, and therefore their binding could be examined using SPR. pra third party risk