WebJan 7, 2024 · In BY-2 cells, phosphinothricin had been already used by Nakamura et al. ( 2010 ), who recommended 2 mg/l PPT for selection. However, this protocol did not work in our hands, and later on, PPT was even considered unsuitable for suspension cultures by some authors (Seifertová et al. 2014 ). Web【技术实现步骤摘要】 一种D ‑ 氨基酸氧化酶突变体及其应用 ： [0001]本专利技术涉及蛋白质工程领域，涉及一种D ‑ 氨基酸氧化酶突变体及其应用。 技术介绍 ： [0002]草铵膦(glufosinate ammonium,phosphinothricin简称PPT)，化学名称为(D，L) ‑2‑ 氨基 ‑4‑ [羟基(甲基)膦酰基]丁酸。 其分子式为：C5H 12 NO4P ...

Selection of BASTA resistant Arabidopsis transformants

Web168 protocolsare often genotypespecific (Widholm, 1995; Somers et al., 2003). Increased soybean transforma-tion efficiency, may be achieved by further optimizing how to sign into my school outlook account

菰抗病候选基因筛选及其转基因水稻白叶枯病抗性分析 - 豆丁网

Web1994; Wan and Lemaux 1994). L-Phosphinothricin (PPT), or the ammonium salt, glufosinate, is the active compound of the non-selective herbicide Basta. L-Phos phinothricin is a glutamate analogue that inhibits glutamine synthetase (Manderscheid and Wild 1986). Glutamine synthetase catalyses an ATP-dependent incor WebThe present invention relates to a method of Agrobaterium-based transformation of a plant comprising: generating a callus explant by, performing callus induction and maintenance in a plant in at least a first solution comprising less than about 100 mg/l of inositol; subjecting the callus explant to a cold treatment in a second solution comprising glutamine; and co … WebWild-type strain 21gr of the green alga Chlamydomonas reinhardtii was resistant to the ammonium salt of l-phosphinothricin (PPT, also called glufosinate), an irreversible inhibitor of glutamine synthetase activity and the main active component of the herbicide BASTA (AgrEvo, Frankfurt am Main, Germany). Under the same conditions, however, this strain … how to sign into meta with headset