Phospho-kinase antibody array

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August undefined, 2024

WebJun 18, 2024 · Here, we conducted integrated analysis of human phospho-kinase antibody array, transcriptome analysis with RNA-seq, and proteomic analysis of protein pulldown to study the molecular detail of PSPC1-potentiated phenotypical transformation, adhesion, and motility in human hepatocellular carcinoma (HCC) cells. We found that PSPC1 … WebApr 11, 2024 · Human Phospho-Kinase Array - Eight nitrocellulose membranes; Part A contains 21 antibodies printed in duplicate, and Part B contains 18 antibodies printed in …

Proteome Profiler Human Phospho-Kinase Array Kit

WebProteome Profiler Human Phospho-RTK Array Kit contains 4 membranes - each spotted in duplicate with 42 different RTK antibodies. The R&D Human Phospho-Receptor Tyrosine Kinase (Phospho-RTK) Array is a rapid, sensitive, and economical tool used to detect changes in phosphorylation between samples. R&D Systems Human Phospho-Receptor … WebMay 11, 2024 · An antibody specific to the phosphorylation-of-interest is added to the membrane. The phosphorylation antibody is then bound by a secondary antibody conjugated to horseradish peroxidase (HRP). Antibody binding is detected when an HRP substrate is added, resulting in chemiluminescence. canature north america inc

Protein kinase - Wikipedia

WebPhospho-Kinase A protein kinase is a kinase enzyme that modifies other molecules, mostly proteins, by chemically adding phosphate groups to them (phosphorylation). … WebSep 1, 2024 · Biochemical methods and double-antibody arrays assessed cell growth, metabolism, and intracellular TGF-β and NF-κB signalling pathways activation. ... A human phospho-kinase array was used to ... WebRayBio ® G-Series Human Receptor Tyrosine Kinase Phosphorylation Antibody Array 1 Kit. Detects 71 Human RTKs. Suitable for all liquid sample types but intended for use with cell and tissue lysates. $480.00 CODE: … canature north american reverse osmosis

A human kinase yeast array for the identification of kinases …

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WebThe Human Phospho-Kinase Array is a rapid, sensitive, and economical tool to simultaneously detect the relative levels of phosphorylation of 37 kinase phosphorylation sites and 2 related total proteins without performing numerous immunoprecipitations and Western blots. ... Each capture antibody was carefully selected using cell lysates prepared … WebApr 12, 2024 · Phospho-Kinase Array is a rapid, sensitive, and economical tool to simultaneously detect the ... Each capture antibody was carefully selected using cell lysates prepared from cell lines known to express the target protein. PRINCIPLE OF THE ASSAY Capture and control antibodies have been spotted in duplicate on nitrocellulose … canature processing langleyWebNational Center for Biotechnology Information fish ick medicine

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Phospho-kinase antibody array

WebProtein kinase. A protein kinase is a kinase which selectively modifies other proteins by covalently adding phosphates to them ( phosphorylation) as opposed to kinases which … WebThe newly updated T-Cell Receptor Phospho Antibody Array is a high-throughput ELISA based antibody array for qualitative protein expression profiling. It is suitable for comparing protein expressions between non-treated and treated samples and identifying candidate biomarkers related to TCR signaling.

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WebMouse RTK Phosphorylation Array C1 RayBio ® C-Series Mouse Receptor Tyrosine Kinase Phosphorylation Antibody Array 1 Kit. Detects 71 Mouse RTKs. Suitable for all liquid sample types but intended for use with cell and tissue lysates. $378.00 CODE: AAM-PRTK-1-2 Number of Membranes: Quantity: + − Add To Cart View all product options Description WebProduct name Human EGFR Phosphorylation Antibody Array - Membrane (17 Targets) Sample type Tissue Extracts, Cell Lysate Assay type Semi-quantitative Species reactivity Reacts with: Human Product overview ab134005 is for simultaneous detection of the relative levels of 17 different Phosphorylated Human EGF Receptors.

WebThe array kit allows for the simultaneous detection of 28 receptor tyrosine kinases and 11 important signaling nodes, when phosphorylated at tyrosine or other residues. Target-specific capture antibodies have been spotted in … WebFigure 1 Detection of phospho-tyrosine growth factor receptors using a dot blot array. Phospho-tyrosine dot blot analyses of esophageal cancer cell lines OE33 and FLO-1. The lower portion of each panel is annotated showing what each positive spot represents. Note each blot has 3 internal reference spots that provide a comparator for comparing ...

WebSep 1, 2024 · Phospho-Protein Arrays as Effective Tools for Screening Possible Targets for Kinase Inhibitors and Their Use in Precision Pediatric Oncology September 2024 Frontiers … WebKinase Antibody Array - Full Moon BioSystems Kinase Antibody Array Kinase Antibody Array features 276 well-characterized kinase and related antibodies associated with …

WebFeb 28, 2024 · To validate the kinase array phospho-Y2H results, AAR2 and PRPF8 were tested in a co-expression and immunoprecipitation assay in HEK293 cells. Co-expression of either SGK2 or CK2α1 reduced the amount of wild-type AAR2 co-immunoprecipitated with PRPF8 in comparison to the nonphosphorylatable mutant S284A (Fig 5F). Together, these …

WebHuman Phospho AKT Pathway Protein Array, 18 Target proteins (HA-PAA-PA2) Human Phospho AKT Pathway Protein Array, 18 Target proteins (HA-PAA-PA2) ... AMPKa (Thr172), S6 Ribosomal Protein (Ser235/236), mTOR (Ser2448), HSP27 (Ser78), Bad (Ser112), p70 S6 Kinase (Thr389), PRAS40 (Thr246), p53 (Ser15), p38 (Thr180/Tyr182), SAPK/JNK … fishick storeWebRayBiotech: Empowering Your Proteomics canature tank frpWebJan 9, 2024 · Phospho-proteomic analyses revealed that, while not impacting on the global tyrosine phospho-proteome in colon cancer cells, this region modulates phosphorylation of specific membrane-localized... fish ick medication fish ick spotsWebR&D Systems' Mouse Phospho-Receptor Tyrosine Kinase (RTK) Array Kit (Catalog # ARY014) is a screening tool designed to simultaneously detect the relative phosphorylation of 39 different RTKs. The Kit eliminates the need for numerous immunoprecipitation (IP) and/or Western blot experiments. canature reverse osmosis filtersWebHuman RTK Phosphorylation Antibody Array -Membrane (71 Targets) Sample type Cell Lysate, Tissue Lysate Assay type Semi-quantitative Species reactivity Reacts with: Human … canature processing ltd langley bcWebHuman Phospho-Kinase Antibody Array (Membrane, 43 Targets) (Y0225) (AbAr-0225-YC) Antibody array is a specific form of protein microarray. In this technology, capture … canature ro water system